alpha Fetoprotein anticorps

N° du produit ABIN3025146

L’anticorps Souris Monoclonal anti-alpha Fetoprotein a été validé pour IF, FACS et IHC (p). Il convient pour détecter alpha Fetoprotein dans des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour alpha Fetoprotein anticorps (ABIN3025146)

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Fetoprotein est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C2

Détail du produit anti-alpha Fetoprotein anticorps

Aucune reactivité croisée: Boeuf (Vache), Chien, Rat (Rattus)

Attributs du produit: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Purification: Protein G affinity chromatography

Immunogène: AFP purified from serum of a hepatoma patient was used as the immunogen for the Alpha Fetoprotein antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Alpha Fetoprotein antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Alpha Fetoprotein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur alpha Fetoprotein

Antigène: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Autre désignation: alpha Fetoprotein

Sujet: It recognizes an oncofetal glycoprotein with a single chain of 70  kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Pathways: C21-Steroid Hormone Metabolic Process